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We're pleased to announce a major DECODR update. This update includes several new features, alongside required user-account registration. To learn more about these features, click here.

DECODR v3.0

Deconvolution of Complex DNA Repair: Analyze gene editing efficiency with Sanger sequencing traces.

DECODR provides a convenient, cost-effective, and user-friendly method to quantify the edits present in CRISPR-edited Sanger sequencing data.

DECODR is capable of analyzing a wide range of edits, including:
  • indels (both single and multi-guide)
  • multi-guide fragment excisions
  • compound indels (combined insertion and deletion events)
  • HDR insertions and single-nucleotide substitutions

Using a computationally-efficient algorithm, DECODR has virtually no limit on indel size (can quantify deletions several hundred bp long) and can determine the identity of inserted bases. DECODR supports batch analysis, in which several sequencing files can be analyzed at a time.

DECODR is to be used for educational purposes only. By proceeding, you agree that your use of DECODR is strictly for educational purposes. If you are interested in any other uses of DECODR, including without limitation, commercial use, please contact ChristianaCare at licensing.decodr@geneeditinginstitute.com.

October 5, 2022

Features

  • Summary spreadsheet included in output report downloads, i.e. the files received when pressing the 'Download all analysis data' button. Summary spreadsheet contains analysis parameters, failed experimental files, analyzed experimental files, Sanger deconvolutions, and additional metadata.

December 16, 2022

Fixes

  • Donor sequences with large insertions now properly align.

December 23, 2022

Fixes

  • Analyses with long filenames (> 30 characters) can now be downloaded; example results also properly download.

FeaturesDECODR (Previous)DECODR (New)
Batch analysis
Bulk analysis of KO indels
Bulk analysis of HDR recombination
Insertion probabilities
Multi-guide analysis
Compound indels (insertions and deletions)
Indel analysis >100bp
Flexible input format (.ab1 vs .txt/.fasta/.ab1)
One-click shareable outputs
Guideless analysis
Paired-end (FWD + REV) combined analysis
Account-based analysis history
Pipeline-friendly batch upload
Sequence chromatogram visualization