DECODR provides a convenient, cost-effective, and user-friendly method to quantify the edits present in CRISPR-edited Sanger sequencing data.
DECODR is capable of analyzing a wide range of edits, including:
- indels (both single and multi-guide)
- multi-guide fragment excisions
- compound indels (combined insertion and deletion events)
- HDR insertions and single-nucleotide substitutions
Using a computationally-efficient algorithm, DECODR has virtually no limit on indel size (can quantify deletions several hundred bp long) and can determine the identity of inserted bases. DECODR supports batch analysis, in which several sequencing files can be analyzed at a time.
To use DECODR, all you need to do is perform standard sequencing reactions on your experimental files. The resultant .ab1 files can be uploaded to DECODR, and after providing either a text or .ab1 file for the reference sequence, submit your files for analysis to receive near NGS-level results! For more information, see our
publication.
Click here to start using DECODR!Email
decodr@geneeditinginstitute.com for access inquiries, bug reports, or any other suggestions.